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KMID : 0624620080410050369
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BMB Reports
2008 Volume.41 No. 5 p.369 ~ p.375
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Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites
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Liu Bo
Xu Xiaochao
Cheng Yan
Huang Jian
Liu Yan-Hong
Liu Zhen
Min Ming-wei
Bian He-Jiao
Chen Jing
Bao Jin Ku
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Abstract
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Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a dose-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL. [BMB reports 2008; 41(5): 369-375]
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KEYWORD
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Apoptosis, Chemical modification, Mannose-bindi ng sites, Polygonatum cyrtonema Lectin (PCL)
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